Maryanski J L, Van Snick J, Cerottini J C, Boon T
Eur J Immunol. 1982 May;12(5):401-6. doi: 10.1002/eji.1830120508.
The cytolytic T lymphocyte (CTL) response of syngeneic mice to antigenic variants obtained by mutagenesis of mastocytoma P815 was analyzed at the clonal level. Estimates of the frequency of CTL precursor cells in spleens from mice immunized with P815 variants ranged from 10(-4) to 2 X 10(-3). This frequency could be increased approximately 100 times by stimulating immune spleen cells in mass culture for 6-8 days with the immunizing variant. Specificity analysis of a large number of individual CTL clones demonstrated the existence of two distinct populations of CTL. Some CTL clones lysed exclusively the immunizing variant, while others lysed equally well all P815 targets, but not syngeneic tumor L1210. These results provide direct evidence for the existence of new, individual specificities on P815 variants in addition to a common antigen already present on the original P815 cells. This confirms that a variety of new antigens can be induced by mutagenesis on the same background cell. Stable, highly active CTL clones specific for either individual variant antigens or common P815 antigens could be maintained in long-term culture.
在克隆水平上分析了同基因小鼠对通过诱变肥大细胞瘤P815获得的抗原变体的细胞溶解性T淋巴细胞（CTL）反应。用P815变体免疫的小鼠脾脏中CTL前体细胞频率估计值在10^(-4)至2×10^(-3)之间。通过用免疫变体在大规模培养中刺激免疫脾细胞6至8天，该频率可增加约100倍。对大量单个CTL克隆的特异性分析表明存在两种不同的CTL群体。一些CTL克隆仅裂解免疫变体，而其他克隆对所有P815靶标裂解效果相同，但对同基因肿瘤L1210无裂解作用。这些结果为P815变体上除了原始P815细胞上已存在的共同抗原之外还存在新的个体特异性提供了直接证据。这证实了在相同背景细胞上通过诱变可以诱导多种新抗原。对单个变体抗原或共同P815抗原具有特异性的稳定、高活性CTL克隆可在长期培养中维持。
