抗侵袭因子（AIF）在牛软骨基质中的超微结构免疫组织化学定位

Ultrastructural immunohistochemical localization of anti-invasion factor (AIF) in bovine cartilage matrix.

作者信息

Wezeman F H, Childs G V

出版信息

J Histochem Cytochem. 1982 Jun;30(6):524-31. doi: 10.1177/30.6.6178780.

DOI:10.1177/30.6.6178780

Abstract

Rabbit antibodies prepared against bovine cartilage anti-invasion factor (AIF) were tested for their affinity toward antigenic sites in glutaraldehyde-fixed bovine hyaline cartilage matrix. Ultrastructural localization of the antigen-antibody complex was accomplished by the unlabeled antibody peroxidase-antiperoxidase staining technique. Unextracted and salt-extracted (1 M NaCl or 3 M GuHCl) cartilage slices were incubated with anti-AIF antibodies at a working dilution of 1:20,000. Staining occurred in unextracted matrix distributed throughout the tissue, but with regional variation in the lacunar matrix. Significantly less stain was noted in extracted tissues. The results suggest that at least certain protein components in AIF are morphologically associated with matrix complexes in aldehyde-fixed tissue.

摘要

针对牛软骨抗侵袭因子（AIF）制备的兔抗体，检测其对戊二醛固定的牛透明软骨基质中抗原位点的亲和力。抗原 - 抗体复合物的超微结构定位通过未标记抗体过氧化物酶 - 抗过氧化物酶染色技术完成。未提取和经盐提取（1 M NaCl或3 M盐酸胍）的软骨切片与工作稀释度为1:20,000的抗AIF抗体孵育。染色出现在分布于整个组织的未提取基质中，但在腔隙基质中有区域差异。在提取的组织中观察到的染色明显较少。结果表明，AIF中至少某些蛋白质成分在形态学上与醛固定组织中的基质复合物相关。