Sefton B M, Patschinsky T, Berdot C, Hunter T, Elliott T
J Virol. 1982 Mar;41(3):813-20. doi: 10.1128/JVI.41.3.813-820.1982.
p60src, the transforming protein of Rous sarcoma virus, was found to contain 0.5 to 0.9 mol of total phosphate per mol of polypeptide. The protein is known to be phosphorylated at two sites, a serine in the amino-terminal domain and a tyrosine in the carboxy-terminal domain. Because our indirect analysis suggests that the serine is phosphorylated to approximately twice the extent of the tyrosine, we estimate that p60src contains approximately 0.3 to 0.6 mol of phosphoserine and 0.2 to 0.3 mol of phosphotyrosine per mol of polypeptide. p60src was found to represent approximately 0.02% of the total incorporated radioactivity in Rous sarcoma virus-transformed chick cells labeled with [35S]methionine for 48 h. This corresponds to approximately 500,000 molecules of p60src per cell. Pulse-chase experiments revealed that the half-life of p60src ranged from 2 to 7 h, depending on the strain of virus examined. The P60src of the Schmidt-Ruppin strain was significantly more stable than that of the Prague strain.
劳氏肉瘤病毒的转化蛋白p60src被发现每摩尔多肽含有0.5至0.9摩尔的总磷酸盐。已知该蛋白在两个位点被磷酸化，一个是氨基末端结构域中的丝氨酸，另一个是羧基末端结构域中的酪氨酸。由于我们的间接分析表明丝氨酸的磷酸化程度约为酪氨酸的两倍，我们估计每摩尔多肽中p60src含有约0.3至0.6摩尔的磷酸丝氨酸和0.2至0.3摩尔的磷酸酪氨酸。在用[35S]甲硫氨酸标记48小时的劳氏肉瘤病毒转化的鸡细胞中，发现p60src约占总掺入放射性的0.02%。这相当于每个细胞约500,000个p60src分子。脉冲追踪实验表明，p60src的半衰期为2至7小时，这取决于所检测的病毒株。施密特-鲁平株的P60src比布拉格株的稳定性明显更高。
