Filippi P, Vigne R, Quérat G, Jouanny C, Sauze N
J Virol. 1982 Jun;42(3):1057-66. doi: 10.1128/JVI.42.3.1057-1066.1982.
Sheep choroid plexus cells infected with visna virus produce intracytoplasmic viral ribonucleoprotein complexes with sedimentation values of 120S to 200S and buoyant densities of 1.29 to 1.32 g/cm3. These ribonucleoprotein complexes display an endogenous RNA-directed DNA polymerase activity and contain all of the species of RNA associated with polysomes. An analysis of the polypeptides present in the ribonucleoproteins allowed us to identify the mature internal virion core proteins and their precursor, Pr55gag, as well as the glycosylated envelope precursor gPr150env and small amounts of mature glycoprotein gp135. Ultracentrifugation-purified ribonucleoproteins could infect sheep choroid plexus cells and led to a normal lytic cycle with virus production. Our results suggest that visna virus can propagate by means of intracellular infectious particles.
感染维斯纳病毒的绵羊脉络丛细胞会产生胞质内病毒核糖核蛋白复合体，其沉降值为120S至200S，浮力密度为1.29至1.32 g/cm³。这些核糖核蛋白复合体表现出内源性RNA指导的DNA聚合酶活性，并含有与多核糖体相关的所有RNA种类。对核糖核蛋白中存在的多肽进行分析，使我们能够鉴定出成熟的病毒内部核心蛋白及其前体Pr55gag，以及糖基化的包膜前体gPr150env和少量成熟糖蛋白gp135。超速离心纯化的核糖核蛋白可感染绵羊脉络丛细胞，并导致产生病毒的正常裂解周期。我们的结果表明，维斯纳病毒可通过细胞内感染性颗粒进行传播。
