Filippi P, Brahic M, Vigne R, Tamalet J
J Virol. 1979 Jul;31(1):25-30. doi: 10.1128/JVI.31.1.25-30.1979.
Visna virus is a retrovirus responsible for a classical slow infection of the central nervous system of sheep. In the present work we focused our attention on the viral mRNA's. We found that, during the acute infection in vitro, (i) viral mRNA's amount to only 0.1% of the total cytoplasmic RNA, (ii) 20% of the total cytoplasmic viral RNA is found in polyribosomes, and (iii) three viral mRNA's can be identified by sucrose gradient sedimentation or polyacrylamide gel electrophoresis. Their sedimentation coefficients are 36S, 27S, and 21S.
维斯纳病毒是一种逆转录病毒，可引发绵羊中枢神经系统典型的慢感染。在本研究中，我们将注意力集中在病毒信使核糖核酸上。我们发现，在体外急性感染期间，（i）病毒信使核糖核酸仅占细胞质总核糖核酸的0.1%，（ii）细胞质中总病毒核糖核酸的20%存在于多核糖体中，并且（iii）三种病毒信使核糖核酸可通过蔗糖梯度沉降或聚丙烯酰胺凝胶电泳鉴定。它们的沉降系数分别为36S、27S和21S。
