维斯纳病毒信使核糖核酸的特性分析
Characterization of visna virus mRNA.
作者信息
Filippi P, Brahic M, Vigne R, Tamalet J
出版信息
J Virol. 1979 Jul;31(1):25-30. doi: 10.1128/JVI.31.1.25-30.1979.
Abstract
Visna virus is a retrovirus responsible for a classical slow infection of the central nervous system of sheep. In the present work we focused our attention on the viral mRNA's. We found that, during the acute infection in vitro, (i) viral mRNA's amount to only 0.1% of the total cytoplasmic RNA, (ii) 20% of the total cytoplasmic viral RNA is found in polyribosomes, and (iii) three viral mRNA's can be identified by sucrose gradient sedimentation or polyacrylamide gel electrophoresis. Their sedimentation coefficients are 36S, 27S, and 21S.
摘要
维斯纳病毒是一种逆转录病毒，可引发绵羊中枢神经系统典型的慢感染。在本研究中，我们将注意力集中在病毒信使核糖核酸上。我们发现，在体外急性感染期间，（i）病毒信使核糖核酸仅占细胞质总核糖核酸的0.1%，（ii）细胞质中总病毒核糖核酸的20%存在于多核糖体中，并且（iii）三种病毒信使核糖核酸可通过蔗糖梯度沉降或聚丙烯酰胺凝胶电泳鉴定。它们的沉降系数分别为36S、27S和21S。
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本文引用的文献
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Visna, a demyelinating transmissible disease of sheep.维斯纳病，一种绵羊的脱髓鞘性传染病。
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Polyadenylic acid in Visna virus RNA.维斯纳病毒核糖核酸中的多聚腺苷酸
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Virus-specific messenger RNA on free and membrane-bound polyribosomes from cells infected with Rauscher leukemia virus.来自感染劳斯氏白血病病毒的细胞的游离及膜结合多核糖体上的病毒特异性信使核糖核酸。
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Characterization of messenger ribonucleoprotein and messenger RNA from KB cells.来自KB细胞的信使核糖核蛋白和信使核糖核酸的特性分析。
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Properties and location of poly(A) in Rous sarcoma virus RNA.劳氏肉瘤病毒RNA中聚腺苷酸（poly(A)）的特性与位置
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