Dembo M, Kagey-Sobotka A, Lichtenstein L M, Goldstein B
Mol Immunol. 1982 Mar;19(3):421-34. doi: 10.1016/0161-5890(82)90208-5.
We present a kinetic model of histamine release from human basophils due to covalently linked IgE dimers. Comparison of theory with experiment shows that the model gives a good description of histamine release by IgE dimers and allows a number of the parameters of the model to be determined. Comparison with previous models of release by conventional antigens indicates that despite their covalent structure, IgE dimers are subject to the same laws governing inactivation as are antigen produced crosslinks. In addition, the kinetic equation which relates the rate of histamine release to the number of crosslinked Fc epsilon receptors per cell is the same for crosslinks formed by IgE dimers as for antigen induced crosslinks. Quantitative fitting of histamine release data also yields a value for the rate constant for crosslink formation by IgE dimer on the cell surface (rx approximately 5 x 10(-10) cm2/sec). This rate constant is remarkably high and indicates that the reaction is diffusion controlled.
我们提出了一个关于共价连接的IgE二聚体导致人嗜碱性粒细胞释放组胺的动力学模型。理论与实验的比较表明，该模型能很好地描述IgE二聚体引起的组胺释放，并能确定模型的一些参数。与先前传统抗原释放模型的比较表明，尽管IgE二聚体具有共价结构，但它们与抗原产生的交联一样，受相同的失活规律支配。此外，将组胺释放速率与每个细胞交联的Fcε受体数量相关联的动力学方程，对于由IgE二聚体形成的交联和抗原诱导的交联是相同的。组胺释放数据的定量拟合还得出了IgE二聚体在细胞表面形成交联的速率常数的值（rx约为5×10^(-10) cm2/秒）。这个速率常数非常高，表明该反应受扩散控制。
