Zhou T, Conroy M C, Spengler H, de Weck A L
Int Arch Allergy Appl Immunol. 1984;74(2):108-12. doi: 10.1159/000233529.
We have examined the ability of IgG subclass antibodies to release histamine from human leukocytes using covalently cross-linked oligomers of human myeloma proteins. Purified IgG1, G2, G3, G4, (or IgE) was incubated with dimethyl suberimidate to induce cross-linking. The resulting dimers, trimers, and higher molecular weight oligomers were isolated using gel filtration columns (Sephadex G200 and Ultrogel AcA 22) connected in tandem. None of the oligomers of IgG1, G2, G3, or G4 released histamine from leukocytes of donors whose basophils released histamine when challenged with IgE dimer. Furthermore, preincubation with subclass specific oligomers did not desensitize cells to challenge with IgE dimer or to anti-IgE. We conclude that, under our experimental conditions, oligomers of human IgG myeloma subclass antibodies do not trigger histamine release nor modulate IgE-mediated reactions.
我们使用人骨髓瘤蛋白的共价交联低聚物，检测了IgG亚类抗体从人白细胞释放组胺的能力。将纯化的IgG1、G2、G3、G4（或IgE）与辛二亚氨酸二甲酯一起孵育以诱导交联。使用串联连接的凝胶过滤柱（葡聚糖G200和琼脂糖凝胶AcA 22）分离得到的二聚体、三聚体和更高分子量的低聚物。当用IgE二聚体攻击时嗜碱性粒细胞会释放组胺的供体白细胞中，IgG1、G2、G3或G4的低聚物均未释放组胺。此外，用亚类特异性低聚物预孵育不会使细胞对IgE二聚体攻击或抗IgE脱敏。我们得出结论，在我们的实验条件下，人IgG骨髓瘤亚类抗体的低聚物不会触发组胺释放，也不会调节IgE介导的反应。
