Scott M L, Merrett T G, Ishizaka K, Thornley M J, Coombs R R
Clin Exp Immunol. 1982 May;48(2):417-22.
Serum IgE levels can be measured by reverse passive antiglobulin haemagglutination (RPAH) using trypsin-treated human red cells coupled to anti-human IgE by chronic chloride. The results are read after only 90 min incubation. RPAH and double antibody radioimmunoassay have been used to measure IgE levels in 100 sera, with levels ranging from 5 to 43,000 international units (i.u.)/ml. Correlation between the two assays was high over the whole range, provided that affinity-purified anti-IgE was used in the RPAH test. When two non-affinity-purified anti-IgE reagents were used in the RPAH, correlation was poor for sera with levels below 1000 i.u./ml. It is concluded that RPAH tests for IgE are of comparable sensitivity and specificity to radioimmunoassay procedures, and provide a useful simple, yet more rapid alternative.
血清IgE水平可以通过反向被动抗球蛋白血凝试验（RPAH）来测量，该试验使用经胰蛋白酶处理的人红细胞，通过慢性氯化物与抗人IgE偶联。仅孵育90分钟后即可读取结果。RPAH和双抗体放射免疫测定法已用于测量100份血清中的IgE水平，水平范围为5至43000国际单位（i.u.）/毫升。如果在RPAH试验中使用亲和纯化的抗IgE，则两种测定法在整个范围内的相关性都很高。当在RPAH中使用两种非亲和纯化的抗IgE试剂时，对于IgE水平低于1000 i.u./毫升的血清，相关性较差。结论是，IgE的RPAH试验在敏感性和特异性方面与放射免疫测定程序相当，并且提供了一种有用的简单但更快速的替代方法。
