Eggert F M, Gurner B W
Infect Immun. 1984 Jun;44(3):660-4. doi: 10.1128/iai.44.3.660-664.1984.
Colostrum or early breast milk or both from each of 16 healthy women contained agglutinating antibodies for all normal streptococcal inhabitants of the human oral cavity (S. mutans, S. sanguis, S. mitis, and S. salivarius), including those which colonize the neonatal oral cavity in significant numbers. Agglutination correlated with the amount of immunoglobulin A (IgA) binding to bacterial surfaces as measured by mixed reverse passive antiglobulin hemagglutination. Surprisingly, colostral IgA agglutinated our control organism, Brucella abortus. Low levels of colostral or milk IgM and IgG antibodies also reacted with all of the test bacteria. Absorption studies with an enzyme-linked immunosorbent assay showed that a proportion of antibodies in colostrum and early milk is specific for each of the different oral streptococci. Fractionation on Sepharose 4B indicated that 11S secretory IgA is the predominant form of colostral and milk antibody for all of the test bacteria, including B. abortus. No evidence was found that reactions other than antigen-antibody reactions resulted in binding of colostral immunoglobulins by any of the test bacteria.
16名健康女性的初乳或早期母乳或两者中均含有针对人类口腔所有正常链球菌定植菌（变形链球菌、血链球菌、缓症链球菌和唾液链球菌）的凝集抗体，包括那些大量定植于新生儿口腔的细菌。凝集作用与通过混合反向被动抗球蛋白血凝试验测定的与细菌表面结合的免疫球蛋白A（IgA）量相关。令人惊讶的是，初乳IgA能凝集我们的对照菌流产布鲁氏菌。初乳或母乳中低水平的IgM和IgG抗体也能与所有测试细菌发生反应。酶联免疫吸附试验的吸收研究表明，初乳和早期母乳中的一部分抗体对每种不同的口腔链球菌具有特异性。在琼脂糖4B上进行分级分离表明，11S分泌型IgA是初乳和母乳中针对所有测试细菌（包括流产布鲁氏菌）的主要抗体形式。未发现有证据表明除抗原 - 抗体反应外的其他反应会导致任何测试细菌结合初乳免疫球蛋白。
