Coombs R R, Edebo L, Feinstein A, Gurner B W
Immunology. 1978 Jun;34(6):1037-44.
A test is described which is capable of differentiating and measuring by titration the individual classes of antibody reacting with a bacterial suspension. The serum or fluid under test is incubated with the bacteria which are then very well washed and added to indicator red cells linked with specific antiglobulin reagents. Sensitization of the bacteria by a particular class of antibody is shown by haemagglutination (passive) of the appropriate red cells. Agglutination of the bacteria themselves does not preclude an analysis. The reaction, which has been developed on a brucella system, has been designated Mixed Reverse Passive Antiglobulin Haemagglutination (or MRPAH for short).
本文描述了一种检测方法,该方法能够通过滴定区分并测量与细菌悬液发生反应的各类抗体。将待测血清或液体与细菌一起孵育,随后对细菌进行充分洗涤,再加入与特定抗球蛋白试剂相连的指示红细胞。特定类别的抗体使细菌致敏可通过相应红细胞的血凝反应(被动)表现出来。细菌自身的凝集并不妨碍分析。该反应是在布鲁氏菌系统上研发出来的,被命名为混合反向被动抗球蛋白血凝反应(简称为MRPAH)。