Cremer C, Gray J W, Ropers H H
Hum Genet. 1982;60(3):262-6. doi: 10.1007/BF00303014.
A Chinese hamster X man hybrid cell line (CH-Y-VII) was established which retains a free human Y chromosome. Exponentially growing CH-Y-VII cells were arrested with colcemid; metaphase chromosomes were isolated and stained with 33258 Hoechst (HO) plus Chromomycin A3 (CA3), or with ethidium bromide (EB). The HO/CA3-stained chromosomes were measured in a dual beam flow cytometer, and bivariate HO/CA3 flow karyotypes and univariate HO and CA3 flow karyotypes were established. EB-stained chromosomes were analyzed in a modified Becton Dickinson FACS-Sorter. For all three stains used, the human Y chromosome forms a separate peak in univariate flow karyotypes; the optimum resolution was obtained for the HO distribution. In the bivariate HO/CA3 flow karyotype, the peak for the human Y chromosome is completely separated from the Chinese hamster chromosomes.
建立了一种保留游离人类Y染色体的中国仓鼠与人类的杂交细胞系（CH-Y-VII）。对数生长期的CH-Y-VII细胞用秋水仙酰胺处理使其停滞；分离中期染色体并用33258 Hoechst（HO）加放线菌素A3（CA3）或溴化乙锭（EB）染色。在双光束流式细胞仪中测量HO/CA3染色的染色体，并建立双变量HO/CA3流式核型和单变量HO和CA3流式核型。在改良的Becton Dickinson FACS-Sorter中分析EB染色的染色体。对于所使用的所有三种染色剂，人类Y染色体在单变量流式核型中形成一个单独的峰；HO分布获得了最佳分辨率。在双变量HO/CA3流式核型中，人类Y染色体的峰与中国仓鼠染色体完全分开。
