Sillar R, Young B D
J Histochem Cytochem. 1981 Jan;29(1):74-8. doi: 10.1177/29.1.6162882.
A new method for the preparation of metaphase chromosomes for flow analysis has been evaluated. It has been shown that this method, which involves detergent lysis of metaphase cells and polyamines to stabilize the DNA, yields lower coefficients of variation and background levels in the DNA histograms than is currently obtained by hexylene glycol based methods. A conventional flow cytometer (FACS-II) has been used to resolve the human karyotype into about 14 peaks after ethidium bromide staining and excitation with a relatively low level of illumination (0.4 W at 488 nm). Flow karyotypes have also been obtained from suspension cell lines, in particular from the mouse cell line, Friend 707/B10. The only disadvantage of this method is that the chromosomes are highly condensed and therefore banding studies on sorted chromosomes may not be possible.
一种用于制备用于流式分析的中期染色体的新方法已得到评估。结果表明，这种方法涉及用去污剂裂解中期细胞并用多胺稳定DNA，与目前基于己二醇的方法相比，在DNA直方图中产生的变异系数和背景水平更低。使用传统的流式细胞仪（FACS-II），在溴化乙锭染色并用相对较低水平的光照（488nm处0.4W）激发后，已将人类核型解析为约14个峰。也已从悬浮细胞系中获得流式核型，特别是从小鼠细胞系Friend 707/B10中获得。这种方法的唯一缺点是染色体高度浓缩，因此对分选的染色体进行显带研究可能无法进行。
