Klinman D M, Smilek D E, McKearn T J
J Immunol. 1982 Sep;129(3):1204-8.
Sixteen Lewis anti-Brown Norway monoclonal antibodies and sera from alloimmunized Lewis rats were used to study the topographic relationships of antigenic determinants on class I major histocompatibility gene products. Only two independent antigenic regions were identified in competition binding assays. The first region is composed of a set of overlapping epitopes that are conserved in class I major histocompatibility products of mice and humans, as well as rats. In contrast, the second antigenic region appears in a restricted number of inbred rat strains and is not detected in other species. The data provide serologic confirmation, at the monoclonal and serum alloantibody level, of conserved polymorphisms in the major histocompatibility gene products of different species, a finding that is consistent with the amino acid sequences of these molecules.
利用来自同种免疫的Lewis大鼠的16种Lewis抗Brown Norway单克隆抗体和血清，研究I类主要组织相容性基因产物上抗原决定簇的拓扑关系。在竞争结合试验中仅鉴定出两个独立的抗原区域。第一个区域由一组重叠表位组成，这些表位在小鼠、人类以及大鼠的I类主要组织相容性产物中保守。相反，第二个抗原区域出现在数量有限的近交大鼠品系中，在其他物种中未检测到。这些数据在单克隆和血清同种异体抗体水平上提供了不同物种主要组织相容性基因产物中保守多态性的血清学证据，这一发现与这些分子的氨基酸序列一致。
