哮喘的巴仙吉犬-灵缇犬模型：白细胞组胺释放、血清IgE以及气道对吸入抗原的反应。

The Basenji-Greyhound dog model of asthma: leukocyte histamine release, serum IgE, and airway response to inhaled antigen.

作者信息

Peters J E, Hirshman C A, Malley A

出版信息

J Immunol. 1982 Sep;129(3):1245-9.

Abstract

To understand the immunologic mechanisms underlying the variation in airway response to inhaled Ascaris antigen (AA) in Basenji-Greyhound (BG) dogs having hyperreactive airways, we examined the relationship between leukocyte histamine release, Ascaris-specific serum IgE, changes in pulmonary resistance (RL), and decreases in dynamic compliance (Cdyn). All Ascaris-sensitive BG dogs showing airway responses to AA aerosol challenge exhibited an antigen dose-dependent release of leukocyte histamine, with total leukocyte histamine ranging from 68 to 123 ng/10(7) cells. Airway response to inhaled antigen more closely correlated with antigen dose releasing 50% total leukocyte histamine (RL, r = 0.94); Cdyn, r = 0.82), than with circulating levels of antigen-specific IgE (RL, r = 0.68; Cdyn, r = 0.69). We conclude that the airway response of sensitized BG dogs to AA inhalations is more dependent on factors affecting mediator release from pulmonary sources than circulating specific reaginic antibody.

摘要

为了解患有高反应性气道的贝生吉犬 - 灵缇犬（BG犬）对吸入蛔虫抗原（AA）的气道反应变化背后的免疫机制，我们研究了白细胞组胺释放、蛔虫特异性血清IgE、肺阻力（RL）变化和动态顺应性（Cdyn）降低之间的关系。所有对蛔虫敏感的BG犬在接受AA气雾剂激发后均表现出气道反应，呈现出抗原剂量依赖性的白细胞组胺释放，白细胞组胺总量为68至123 ng/10(7) 个细胞。与抗原特异性IgE的循环水平相比，吸入抗原的气道反应与释放50%白细胞组胺总量的抗原剂量相关性更强（RL，r = 0.94；Cdyn，r = 0.82），而与抗原特异性IgE的循环水平相关性较弱（RL，r = 0.68；Cdyn，r = 0.69）。我们得出结论，致敏BG犬对吸入AA的气道反应更依赖于影响肺源性介质释放的因素，而非循环中的特异性反应素抗体。