人β干扰素的放射免疫测定

Radioimmunoassay of human beta interferon.

作者信息

Neurath A R, Strick N, Raj N B, Pitha P M

出版信息

J Interferon Res. 1982;2(1):51-7. doi: 10.1089/jir.1982.2.51.

DOI:10.1089/jir.1982.2.51

Abstract

A solid phase radioimmunoassay (RIA) for human fibroblast-derived beta (beta) interferon is described using dinitrophenylated (DNP) IgG from rabbit anti-interferon serum and quantitation of the antigen-bound antibodies by subsequently measuring the attachment of 125I-labeled anti-DNP. The sensitivity limit of the assay is 50 u of interferon corresponding to 0.5 ng of interferon protein. Preparations of alpha leukocyte-derived interferon show only marginal cross-reactivity (less than 0.01%) while the mouse interferons (mixture of alpha and beta)are negative in this assay. The simplicity, reproducibility and the possibility of using 125I-labeled anti-DNP as a universal reagent for different anti-interferon sera may widen the development of RIA tests for interferons both in research and clinical areas.

摘要

本文描述了一种用于检测人成纤维细胞来源的β干扰素的固相放射免疫测定法（RIA），该方法使用兔抗干扰素血清中的二硝基苯基化（DNP）IgG，并通过随后测量125I标记的抗DNP的附着来定量抗原结合抗体。该测定法的灵敏度极限为50单位干扰素，相当于0.5纳克干扰素蛋白。α白细胞来源的干扰素制剂仅显示出微量交叉反应性（小于0.01%），而小鼠干扰素（α和β的混合物）在此测定中呈阴性。该方法的简便性、可重复性以及使用125I标记的抗DNP作为不同抗干扰素血清通用试剂的可能性，可能会拓宽干扰素RIA检测在研究和临床领域的发展。