通过放射免疫测定法定量一种高度免疫原性的白细胞抗原（L1）：方法学评估。

Quantitation of a highly immunogenic leukocyte antigen (L1) by radioimmunoassay: methodological evaluation.

作者信息

Dale I, Fagerhol M K, Frigård M

出版信息

J Immunol Methods. 1983 Dec 16;65(1-2):245-55. doi: 10.1016/0022-1759(83)90321-6.

Abstract

This paper describes a radioimmunoassay (RIA) for the quantitation of a major protein antigen (L1) released from human granulocytes. A protein A-carrying strain of Staphylococcus aureus was used as a solid phase IgG-binding reagent for the separation of antigen-antibody complexes from free antigen in the assay. A two-step version of the RIA may be completed in 2.5 h and has a detection limit of about 5 ng/ml. The sensitivity may be increased by minor modifications. A single step version takes 1.5 h and has adequate sensitivity to cover clinically important plasma levels. Selection and optimal use of reagents for the two-step procedure is described and their storage discussed. The simplicity of the procedure combined with high sensitivity and satisfactory accuracy, precision and reproducibility makes this assay suitable for quantitation of L1 in various body fluids.

摘要

本文描述了一种用于定量检测人粒细胞释放的主要蛋白质抗原（L1）的放射免疫分析（RIA）方法。在该分析中，一株携带蛋白A的金黄色葡萄球菌被用作固相IgG结合试剂，用于从游离抗原中分离抗原-抗体复合物。两步法RIA可在2.5小时内完成，检测限约为5 ng/ml。通过微小修改可提高灵敏度。一步法需1.5小时，具有足够的灵敏度以涵盖临床上重要的血浆水平。文中描述了两步法试剂的选择和最佳使用方法，并讨论了其储存条件。该方法操作简单，具有高灵敏度以及令人满意的准确性、精密度和重现性，适用于定量检测各种体液中的L1。