Papkoff J, Hunter T
J Virol. 1982 Aug;43(2):533-43. doi: 10.1128/JVI.43.2.533-543.1982.
The gene products of Gazdar murine sarcoma virus (Gz-MuSV) were identified by in vitro translation of Gz-MuSV virion RNA. An overlapping set of proteins with approximate molecular weights of 37,000 (37K), 33K, 24K, and 18K were synthesized from the transforming gene of Gz-MuSV, v-mosGz. In addition, Gz-MuSV-specific RNA directed the in vitro synthesis of a 62K gag gene protein and a 37.5K env gene-related product. The Gz-MuSV-specific in vitro translation products were compared with the in vitro translation products of M-MuSV 124, an independent isolate with a similar v-mos gene. This analysis showed that the 62K Gz-MuSV gag gene protein and the 37K, 33K, 24K, and 18K v-mosGz proteins were almost identical to the M-MuSV 124 62K (gag) and 37K, 33K, 24K, and 18K (v-mosMo) proteins that we previously identified and characterized. The 37.5K env gene product from Gz-MuSV does not have a correlate in the M-MuSV 124 translation products. These results were analyzed in the context of expectations based on similarities and differences in genetic organization of these two viral genomes.
通过对加兹达尔鼠肉瘤病毒（Gz-MuSV）病毒粒子RNA进行体外翻译，鉴定了该病毒的基因产物。从Gz-MuSV的转化基因v-mosGz合成了一组重叠的蛋白质，其近似分子量分别为37000（37K）、33K、24K和18K。此外，Gz-MuSV特异性RNA指导了62K gag基因蛋白和37.5K env基因相关产物的体外合成。将Gz-MuSV特异性体外翻译产物与M-MuSV 124（一种具有相似v-mos基因的独立分离株）的体外翻译产物进行了比较。该分析表明，62K Gz-MuSV gag基因蛋白以及37K、33K、24K和18K v-mosGz蛋白与我们之前鉴定和表征的M-MuSV 124 62K（gag）和37K、33K、24K和18K（v-mosMo）蛋白几乎相同。Gz-MuSV的37.5K env基因产物在M-MuSV 124翻译产物中没有对应物。基于这两种病毒基因组遗传组织的异同，在预期的背景下对这些结果进行了分析。
