Cremer K, Reddy E P, Aaronson S A
J Virol. 1981 May;38(2):704-11. doi: 10.1128/JVI.38.2.704-711.1981.
In vitro translation of virion RNA of Moloney murine sarcoma virus (MSV) strain 124 yielded major products having molecular weights of 63,000 (63K), 43K, 40K, 31K, and 24K daltons. A molecularly cloned subgenomic fragment of Moloney MSV comprised of the cellular insertion (src) region was utilized in hybridization arrest translation as a means of identifying products of the MSV src gene. MSV src DNA specifically inhibited synthesis of the 43K, 40K, 31K, and 24K proteins, implying that each of these proteins was coded within the MSV src gene. The MSV src-specific nature of this family of proteins was further confirmed by partial purification of MSV src-containing RNAs from MSV non-producer cells. In vitro translation of enriched cellular RNAs yielded products with molecular weights identical to those of the 43K family of proteins synthesized from virion RNA. Nucleotide sequence analysis of the MSV transforming region has revealed a long open reading frame which includes five methionine codons (Reddy et al., Proc. Natl. Acad. Sci. U.S.A. 77:5234-5238, 1980). The molecular weights of the four largest proteins that could be synthesized within this open reading frame corresponded closely to the molecular weights of the 43K family of proteins. Partial cyanogen bromide cleavage of each of the three largest proteins resulted in an uncleaved fragment having a molecular weight equal to that of the smallest (24K) protein. These findings provide direct biochemical evidence that the 43K, 40K, 31K, and 24K proteins are related in their carboxy-terminal regions, as well as information concerning the MSV src gene coding sequences from which each protein originates:
莫洛尼氏鼠肉瘤病毒（MSV）124株病毒粒子RNA的体外翻译产生了分子量分别为63,000（63K）、43K、40K、31K和24K道尔顿的主要产物。由细胞插入（src）区域组成的莫洛尼氏MSV的分子克隆亚基因组片段被用于杂交抑制翻译，作为鉴定MSV src基因产物的一种手段。MSV src DNA特异性抑制了43K、40K、31K和24K蛋白质的合成，这意味着这些蛋白质中的每一种都是由MSV src基因编码的。从MSV非生产细胞中部分纯化含MSV src的RNA，进一步证实了这一族蛋白质的MSV src特异性。富集的细胞RNA的体外翻译产生的产物，其分子量与从病毒粒子RNA合成的43K蛋白质家族的分子量相同。MSV转化区的核苷酸序列分析揭示了一个长的开放阅读框，其中包括五个甲硫氨酸密码子（雷迪等人，《美国国家科学院院刊》77:5234 - 5238，1980）。在这个开放阅读框内能够合成的四种最大蛋白质的分子量与43K蛋白质家族的分子量密切对应。对三种最大蛋白质中的每一种进行部分溴化氰裂解，产生了一个未裂解的片段，其分子量与最小的（24K）蛋白质的分子量相等。这些发现提供了直接的生化证据，表明43K、40K、31K和24K蛋白质在其羧基末端区域是相关的，同时也提供了关于每种蛋白质起源的MSV src基因编码序列的信息：
