Donoghue D J, Hunter T
J Virol. 1983 Feb;45(2):607-17. doi: 10.1128/JVI.45.2.607-617.1983.
Different variants of Moloney murine sarcoma virus (MSV) were examined by nucleotide sequencing to compare the junctions between the acquired cellular sequence, v-mos, and the adjacent virus-derived sequences. These variants included 124-MSV, m1-MSV, and HT1-MSV and also the purportedly independent isolate Gazdar MSV. These four strains have an identical 5' junction between the murine leukemia virus env gene and the v-mos gene. This junction lies within the sixth codon of the chimeric env-mos coding region that encodes the transforming gene product. In contrast, at the 3' junction between the v-mos gene and the murine leukemia virus env gene, the three variants examined here were all different. A small deletion was found in the COOH-terminal portion of the m1-MSV env-mos coding region, indicating that the COOH terminus of this transforming gene product must be different from that of 124-MSV or HT1-MSV. The data presented here are consistent with the thesis that a virus closely related to HT1-MSV was the primordial Moloney MSV, and that all other related strains evolved from it by deletion or rearrangement. The variability observed in the Moloney MSV family is discussed in terms of possible mechanisms for the initial capture of mos sequences by the parental retrovirus and also in comparison with other transforming retrovirus families, such as Abelson murine leukemia virus and Rous sarcoma virus.
通过核苷酸测序对莫洛尼鼠肉瘤病毒（MSV）的不同变体进行了检测，以比较获得的细胞序列v-mos与相邻病毒衍生序列之间的连接。这些变体包括124-MSV、m1-MSV和HT1-MSV，以及据称独立分离的加兹达尔MSV。这四株病毒在鼠白血病病毒env基因和v-mos基因之间具有相同的5'连接。该连接位于编码转化基因产物的嵌合env-mos编码区的第六个密码子内。相比之下，在v-mos基因和鼠白血病病毒env基因之间的3'连接处，这里检测的三个变体均不相同。在m1-MSV env-mos编码区的COOH末端部分发现了一个小缺失，表明该转化基因产物的COOH末端必定与124-MSV或HT1-MSV的不同。此处呈现的数据与以下论点一致：与HT1-MSV密切相关的一种病毒是原始的莫洛尼MSV，所有其他相关毒株均由其通过缺失或重排进化而来。从亲本逆转录病毒最初捕获mos序列的可能机制方面，以及与其他转化逆转录病毒家族（如阿贝尔森鼠白血病病毒和劳氏肉瘤病毒）相比较，对莫洛尼MSV家族中观察到的变异性进行了讨论。
