Mandl J, Mészáros K, Antoni F, Spolarics Z, Garzó T
Mol Cell Biochem. 1982 Jul 7;46(1):25-30. doi: 10.1007/BF00215578.
The inhibition of RNA synthesis of isolated mouse liver parenchymal cells caused by 10 mM D-galactosamine was reversible, while the inhibition of protein synthesis remained unaltered after the removal of galactosamine. 10(-5)M epinephrine and 10(07)M glucagon have been shown to decrease aminoglycogen formation and thus to reduce the inhibitory effect of galactosamine on protein synthesis (II). However, these hormones did not decrease the inhibition of RNA synthesis. 10 mM D-galactosamine did not effect the nucleoside and amino acid incorporation of isolated non-parenchymal mouse liver cells. The predominant role of aminoglycogen in the inhibition of protein synthesis in galactosamine induced liver injury is discussed.
10毫摩尔D - 半乳糖胺对分离的小鼠肝脏实质细胞RNA合成的抑制作用是可逆的，而去除半乳糖胺后蛋白质合成的抑制作用仍未改变。已表明10⁻⁵摩尔/升肾上腺素和10⁻⁷摩尔/升胰高血糖素可减少氨基糖原的形成，从而降低半乳糖胺对蛋白质合成的抑制作用（II）。然而，这些激素并未降低对RNA合成的抑制作用。10毫摩尔D - 半乳糖胺对分离的非实质小鼠肝细胞的核苷和氨基酸掺入没有影响。文中讨论了氨基糖原在半乳糖胺诱导的肝损伤中对蛋白质合成抑制的主要作用。
