Störmer U, Schmidt R, Ruprecht J, Störmer P
Acta Histochem. 1982;70(2):161-72.
Paraffin-embedded human and animal tissues were stained with spectrally pure thionine, 1,3-diaminophenthiazoniumchlorate and 1,7-diaminophenthiazoniumperchlorate. They were examined by light microscopy to determine their applicability in histologic diagnosis. Furthermore the effect of salts and of the type of dye solvent on the staining properties of histological preparations was being investigated. Essentially the following results were obtained: 1. All 3 dyes can be applied rapidly and unproblematically on all types of tissues. Under standardized conditions, i.e. dye concentration, length of staining time and kind of staining procedure, the results are reproducable. 2. Thionine and its isomeres, being basic dyes, demonstrate a special affinity for nuclei, but also do stain cytoplasm sufficiently well to make counter-staining methods unnecessary. 3. Thionine -- in contrary to its isomeres -- reacts metachromatically with certain cellular structural elements, i.e. RNA, acid mucopolysaccharides, mucin. This property aids in morphological diagnosis and additionally enlightens certain activity states. 4. Addition of salts interferes with the staining procedure. 5. Water has special significance as solvent. Furthermore the appearance and function of metachromasia, the influence of ethanol on the metachromatic reaction, the mode of action of anorganic salts are being discussed as well as the reciprocal action of biologic substrates with the dyes having been incorporated by them.
将石蜡包埋的人体和动物组织用光谱纯硫堇、1,3 - 二氨基吩噻嗪氯酸盐和1,7 - 二氨基吩噻嗪高氯酸盐进行染色。通过光学显微镜对其进行检查，以确定它们在组织学诊断中的适用性。此外，还在研究盐类和染料溶剂类型对组织学制剂染色特性的影响。基本上获得了以下结果：1. 所有这三种染料都可以快速且毫无问题地应用于所有类型的组织。在标准化条件下，即染料浓度、染色时间长度和染色程序种类，结果是可重复的。2. 硫堇及其异构体作为碱性染料，对细胞核表现出特殊亲和力，但对细胞质的染色也足够好，无需进行复染方法。3. 硫堇——与其异构体相反——与某些细胞结构成分，即RNA、酸性粘多糖、粘蛋白发生异染反应。这一特性有助于形态学诊断，并进一步揭示某些活性状态。4. 添加盐会干扰染色程序。5. 水作为溶剂具有特殊意义。此外，还讨论了异染现象的外观和功能、乙醇对异染反应的影响、无机盐的作用方式以及生物底物与它们所结合的染料之间的相互作用。
