Mullaney P F, Mann R, Seger G H, Achatz M
Anal Quant Cytol. 1982 Jun;4(2):115-22.
We conducted high-resolution fluorescent image analysis with mithramycin-stained cells from clinical gynecologic specimens. Features characteristic of the usual, low-resolution, one-dimensional, slit-scan flow cytometric measurements were extracted from 250 high-resolution nuclear images. In addition to the measurement of the usual parameters, nuclear ellipticity and DNA density (DNA per unit nuclear size) were also determined. Our preliminary results indicate that both of these features offer increased discrimination. When nuclear shape was included as a global feature, at least 77% of the diagnostic cells could be distinguished from normal cells, with no overlap. Both features hold promise for improving the discrimination possible with flow cytometry.
我们对来自临床妇科标本的光神霉素染色细胞进行了高分辨率荧光图像分析。从250张高分辨率核图像中提取了常规低分辨率一维狭缝扫描流式细胞术测量所具有的特征。除了测量常规参数外，还测定了核椭圆率和DNA密度（单位核大小的DNA）。我们的初步结果表明，这两个特征都能提高鉴别能力。当将核形状作为一个整体特征纳入时，至少77%的诊断细胞能够与正常细胞区分开来，且无重叠。这两个特征都有望改善流式细胞术的鉴别能力。
