免疫组织化学揭示病毒诱导的糖尿病牛外分泌胰腺酶的变化。

Alterations of exocrine pancreatic enzymes in virus-induced diabetic cattle as revealed by immunohistochemistry.

作者信息

Bendayan M, Ito S, Manocchio I

出版信息

Diabetologia. 1982 Jul;23(1):65-8. doi: 10.1007/BF00257734.

Abstract

The pancreatic tissue of normal and virus-induced diabetic cattle was investigated by the indirect immunofluorescence technique. Seven secretory proteins (chymotrypsinogen A, trypsinogen, carboxypeptidase A, RNase, DNase, alpha-amylase and lipase) were localized in normal bovine pancreatic acinar cells but in diabetic animals amylase, lipase and carboxypeptidase were either not detectable or markedly diminished. Decrease in amylase content has been reported previously in other diabetic animals. The diminution of the three pancreatic enzymes may be related to the destruction of pancreatic endocrine tissue that occurs in these diabetic animals.

摘要

采用间接免疫荧光技术对正常牛和病毒诱导的糖尿病牛的胰腺组织进行了研究。七种分泌蛋白（胰凝乳蛋白酶原A、胰蛋白酶原、羧肽酶A、核糖核酸酶、脱氧核糖核酸酶、α淀粉酶和脂肪酶）在正常牛胰腺腺泡细胞中定位，但在糖尿病动物中，淀粉酶、脂肪酶和羧肽酶要么无法检测到，要么显著减少。先前在其他糖尿病动物中也报道过淀粉酶含量的降低。这三种胰腺酶的减少可能与这些糖尿病动物中发生的胰腺内分泌组织破坏有关。