Leeson T S, Higgs G W
Histochem J. 1982 Jul;14(4):553-60. doi: 10.1007/BF01011888.
Results obtained after the normal aldehyde fixation of duodenal enterocytes for electron microscopy have been compared with results obtained when 0.1% Malachite Green or 10 mM lanthanum chloride had been added during aldehyde fixation. Sections were examined without further staining, and after counterstaining with lead citrate and uranyl acetate. In unstained sections, lanthanum-treated material showed improved contrast when compared to results from the other two methods. Also, after counterstaining, areas showing excellent contrast were much more frequent and more readily detected in the lanthanum-treated material. In the microvilli of enterocytes fixed in the presence of lanthanum, the plasmalemma-glycocalyx was defined more clearly and the results were more pleasing subjectively. When Malachite Green was present in the fixative, good contrast was observed more frequently than in routinely fixed tissues, but less often than in those treated with lanthanum. It is suggested that the addition of lanthanum chloride or Malachite Green to the fixative may prove useful in many ultrastructural studies.
将十二指肠肠上皮细胞经正常醛类固定用于电子显微镜观察所获得的结果，与在醛类固定过程中添加0.1%孔雀绿或10 mM氯化镧后所获得的结果进行了比较。切片未经进一步染色以及用柠檬酸铅和醋酸铀酰复染后进行检查。在未染色的切片中，与其他两种方法的结果相比，经镧处理的材料显示出更好的对比度。此外，复染后，在经镧处理的材料中，显示出极佳对比度的区域更为常见且更容易检测到。在镧存在下固定的肠上皮细胞的微绒毛中，质膜-糖萼界定得更清晰，主观上结果也更令人满意。当固定剂中存在孔雀绿时，观察到良好对比度的频率比常规固定组织更高，但比用镧处理的组织更低。有人认为，在固定剂中添加氯化镧或孔雀绿可能在许多超微结构研究中证明是有用的。
