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An automated fluorimetric assay for the simultaneous determinations of histamine and 5-hydroxytryptamine released from rat mast cell preparations.

作者信息

Parsons J F, Safford R J, Philp J

出版信息

J Immunol Methods. 1980;33(2):183-93. doi: 10.1016/s0022-1759(80)80008-1.

DOI:10.1016/s0022-1759(80)80008-1
PMID:6768805
Abstract

An automated assay suitable for the extraction and simultaneous fluorimetric determination of histamine and 5-hydroxytryptamine (5-HT) released from rat isolated peritoneal mast cells is described. The method gave good reproducibility and accuracy in use and required a sample volume of 0.5 ml for assay at a sampling rate of 30/h. The system was evaluated by assessment of its performance in the determination of histamine and 5-HT released from rat mast cells in response to compound 48/80. The detection limit for the procedure was 2 ng 5-HT base/ml and 20 ng histamine base/ml.

摘要

相似文献

1
An automated fluorimetric assay for the simultaneous determinations of histamine and 5-hydroxytryptamine released from rat mast cell preparations.
J Immunol Methods. 1980;33(2):183-93. doi: 10.1016/s0022-1759(80)80008-1.
2
Comparison of histamine and 5-hydroxytryptamine content and secretion in rat mast cells isolated from different anatomical locations.从不同解剖部位分离的大鼠肥大细胞中组胺和5-羟色胺含量及分泌的比较。
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Simultaneous detection of histamine release and lactate production in rat mast cells induced by compound 48/80 using 1H NMR.使用核磁共振氢谱(1H NMR)同时检测化合物48/80诱导大鼠肥大细胞中组胺释放和乳酸生成的情况。
Exp Cell Res. 1991 Apr;193(2):279-82. doi: 10.1016/0014-4827(91)90097-e.

引用本文的文献

1
The effect of adrenocorticotropin on histamine and 5-hydroxytryptamine secretion from rat mast cells.促肾上腺皮质激素对大鼠肥大细胞组胺和5-羟色胺分泌的影响。
Agents Actions. 1984 Apr;14(3-4):454-7. doi: 10.1007/BF01973848.
2
Compound 48/80 and substance P induced release of histamine and serotonin from rat peritoneal mast cells.化合物48/80和P物质诱导大鼠腹膜肥大细胞释放组胺和5-羟色胺。
Agents Actions. 1983 Apr;13(2-3):138-41. doi: 10.1007/BF01967317.