Effects of smoking on platelets and on plasma thromboxane-prostacyclin balance in man.

To determine the influence of brief period of smoking on platelet release and platelet-vessel wall prostaglandins, we studied 13 non-smokers and 15 habitual smokers. Blood samples were collected before and immediately after smoking. Before smoking plasma beta-thromboglobulin levels were similar in the two groups. Plasma levels of thromboxane B2 (TXB2), stable metabolite of TXA2 were slightly higher (124 +/- 43 vs 101 +/- 36 pg/ml) and of 6-keto-PGF1 alpha (stable metabolite of PGI2) lower (39 +/- 23 vs 63 +/- 23 pg/ml) in smokers compared to non-smokers. Immediately after smoking, plasma TXB2 levels increased 197% (P less than 0.05) and plasma 6-keto-PGF1 alpha 44% (P-NS) in non-smokers. Similar increases were not observed in the smoker population, although plasma beta-thromboglobulin levels increased 40%. Platelet TXA2 generations induced by arachidonic acid and thrombin were similar in the two groups. These data show that brief period of smoking stimulates both TXA2 and PGI2 release in the non-smokers. Increase in TXA2 is more than in PGI2. Episodic increase in TXA2 may be a mechanism of vascular injury. Absence of increase in TXA2 and PGI2 with smoking in habitual smokers may reflect tolerance to effects of smoking.