Clark J G, Kuhn C
Am Rev Respir Dis. 1982 Oct;126(4):737-9. doi: 10.1164/arrd.1982.126.4.737.
The development of pulmonary fibrosis in hamsters after the intratracheal instillation of bleomycin is attended by exudation of polymorphonuclear leukocytes (PMNs), but the function of these cells is unknown. In this study, we examined the effect of PMN depletion on lung collagen synthetic rate in bleomycin-treated hamsters. Antiserum to hamster PMNs was produced in rabbits and injected intraperitoneally into hamsters on the fourth through seventh days after intratracheal instillation of bleomycin or saline. Control animals received either normal rabbit serum (NRS) or no serum. Anti-PMN serum (APS) significantly reduced PMN exudation after bleomycin treatment through the eighth day (p less than 0.01). Collagen synthetic rate measured in lung explant cultures was increased in all bleomycin-treated groups compared with that in the saline injected control group (p less than 0.005). Collagen synthesis was greater in neutrophil-depleted bleomycin-treated hamsters than in bleomycin-treated animals receiving NRS or no serum on Days 8 (p less than 0.01) and 12 (p less than 0.05). In APS-treated hamsters, the proportion of protein synthesis directed toward collagen synthesis was increased above other bleomycin-treated groups, suggesting that collagen synthesis is selectively increased in neutrophil-depleted hamsters with bleomycin-induced pulmonary fibrosis.
经气管内注入博来霉素后，仓鼠发生肺纤维化的过程中伴有多形核白细胞（PMNs）渗出，但这些细胞的功能尚不清楚。在本研究中，我们检测了PMNs耗竭对博来霉素处理的仓鼠肺胶原合成率的影响。用兔制备抗仓鼠PMNs抗血清，并在气管内注入博来霉素或生理盐水后的第4至7天腹腔注射到仓鼠体内。对照动物接受正常兔血清（NRS）或不接受血清。抗PMNs血清（APS）显著降低了博来霉素处理后至第8天的PMNs渗出（p<0.01）。与注射生理盐水的对照组相比，所有博来霉素处理组肺外植体培养物中测得的胶原合成率均升高（p<0.005）。在第8天（p<0.01）和第12天（p<0.05），中性粒细胞耗竭的博来霉素处理仓鼠的胶原合成高于接受NRS或不接受血清的博来霉素处理动物。在APS处理的仓鼠中，导向胶原合成的蛋白质合成比例高于其他博来霉素处理组，这表明在博来霉素诱导的肺纤维化的中性粒细胞耗竭仓鼠中，胶原合成选择性增加。
