膜蛋白在苯基硼酸琼脂糖上的分级分离。
Fractionation of membrane proteins on phenylboronic acid-agarose.
作者信息
Williams G T, Johnstone A P, Dean P D
出版信息
Biochem J. 1982 Jul 1;205(1):167-71. doi: 10.1042/bj2050167.
Abstract
Detergent-solubilized plasma-membrane proteins from lymphocytes of patients with chronic lymphocytic leukaemia were applied to phenylboronic acid-agarose. Some of the polypeptides were bound specifically and subsequently eluted with sorbitol. Immunoprecipitation analyses showed that no surface immunoglobulin M was bound, but that most of the histocompatibility antigens HLA-A, HLA-B, HLA-C and HLA-DR were.
摘要
将慢性淋巴细胞白血病患者淋巴细胞经去污剂溶解的质膜蛋白应用于苯硼酸琼脂糖。一些多肽被特异性结合，随后用山梨醇洗脱。免疫沉淀分析表明，表面免疫球蛋白M未被结合，但大多数组织相容性抗原HLA - A、HLA - B、HLA - C和HLA - DR被结合。
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