乙肝表面抗原在与琼脂糖相连的1,9-二氨基壬烷或1,10-二氨基癸烷上的疏水层析。
Hydrophobic chromatography of hepatitis B surface antigen on 1,9-diaminononane or 1,10-diaminodecane linked to agarose.
作者信息
Neurath A R, Lerman S, Chen M, Prince A M
出版信息
J Gen Virol. 1975 Aug;28(2):251-4. doi: 10.1099/0022-1317-28-2-251.
PMID:1176967
Abstract
A series of omega-aminoalkyl-agarose differing in the number of carbon atoms in the alkyl chains was prepared and used for chromatography of hepatitis B surface antigen (HBSAg). HBSAg was separated from the major part of serum proteins by adsorption to columns of 1,9-diaminononane or 1,10-diaminodecane linked to agarose beads (Sepharose 4B or 2B) followed by elution with 4 M-NaSCN.
摘要
制备了一系列烷基链中碳原子数不同的ω-氨基烷基琼脂糖，并将其用于乙型肝炎表面抗原（HBSAg）的色谱分析。通过吸附到与琼脂糖珠（Sepharose 4B或2B）相连的1,9-二氨基壬烷或1,1,0-二氨基癸烷柱上，然后用4M-NaSCN洗脱，将HBSAg与大部分血清蛋白分离。
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