Kay J E, Benzie C R
Biochim Biophys Acta. 1982 Aug 30;698(2):218-21. doi: 10.1016/0167-4781(82)90139-7.
Reticulocyte lysates depleted of mRNA by digestion with micrococcal nuclease still show an unexpectedly high rate of formation of 80 S initiation complexes. Formation of these complexes is sensitive to all inhibitors of the normal protein synthesis initiation process tested. Such lysates contain high concentrations of mRNA fragments which can be utilized for initiation, with which exogenous mRNA must compete. As a consequence of this competition, mRNAs that are weak initiators may be translated poorly by this system even at low exogenous mRNA concentrations.
用微球菌核酸酶消化去除mRNA的网织红细胞裂解物仍显示出80S起始复合物形成的意外高比率。这些复合物的形成对所测试的正常蛋白质合成起始过程的所有抑制剂敏感。此类裂解物含有高浓度的可用于起始的mRNA片段，外源性mRNA必须与之竞争。由于这种竞争，即使在外源性mRNA浓度较低时，作为弱起始子的mRNA也可能被该系统低效翻译。
