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1
Inhibition of eukaryotic protein chain initiation by vanadate.钒酸盐对真核生物蛋白质链起始的抑制作用。
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3148-52. doi: 10.1073/pnas.80.11.3148.
2
Functional relationships between a reticulocyte polypeptide-chain-initiation factor (IF-MP) and the translational inhibitor involved in regulation of protein synthesis by haemin.网织红细胞多肽链起始因子（IF-MP）与参与血红素调节蛋白质合成的翻译抑制剂之间的功能关系。
Eur J Biochem. 1976 Jul 1;66(2):413-22. doi: 10.1111/j.1432-1033.1976.tb10531.x.
3
Regulation of protein synthesis in rabbit reticulocyte lysates: additional initiation factor required for formation of ternary complex (eIF-2.GTP.Met-tRNAf) and demonstration of inhibitory effect of heme-regulated protein kinase.兔网织红细胞裂解物中蛋白质合成的调控：三元复合物（eIF-2·GTP·Met-tRNAf）形成所需的额外起始因子以及血红素调节蛋白激酶抑制作用的证明。
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1079-83. doi: 10.1073/pnas.76.3.1079.
4
The binding of Met-tRNAf to isolated 40-S ribosomal subunits and the formation of Met-tRNAf - 80-S-ribosome initiation complexes.甲硫氨酰 - 起始tRNA与分离的40 - S核糖体亚基的结合以及甲硫氨酰 - 起始tRNA - 80 - S核糖体起始复合物的形成。
Eur J Biochem. 1976 Feb 16;62(2):243-55. doi: 10.1111/j.1432-1033.1976.tb10154.x.
5
Initiation of the polypeptide chain by reticulocyte cell-free systems. Survey of different inhibitors of translation.网织红细胞无细胞系统引发多肽链。翻译的不同抑制剂研究。
Eur J Biochem. 1976 Sep 15;68(2):355-64. doi: 10.1111/j.1432-1033.1976.tb10822.x.
6
Effect of Met-tRNAf deacylase on polypeptide chain initiation in rabbit reticulocyte lysate.甲硫氨酰 - 起始tRNA去酰基酶对兔网织红细胞裂解物中多肽链起始的影响。
J Biol Chem. 1982 May 25;257(10):5722-9.
7
Protein synthesis in rabbit reticulocytes: mechanism of protein synthesis inhibition by heme-regulated inhibitor.兔网织红细胞中的蛋白质合成：血红素调节抑制剂对蛋白质合成的抑制机制。
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5076-9. doi: 10.1073/pnas.76.10.5076.
8
Regulation of protein synthesis in rabbit reticulocyte lysates: characteristics of inhibition of protein synthesis by a translational inhibitor from heme-deficient lysates and its relationship to the initiation factor which binds Met-tRNAf.兔网织红细胞裂解物中蛋白质合成的调控：血红素缺乏裂解物中的翻译抑制剂对蛋白质合成的抑制特性及其与结合甲硫氨酰 - tRNAf的起始因子的关系
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2720-4. doi: 10.1073/pnas.73.8.2720.
9
Protein synthesis in rabbit reticulocytes: a study of the mechanism of action of the protein factor RF that reverses protein synthesis inhibition in heme-deficient reticulocyte lysates.兔网织红细胞中的蛋白质合成：对蛋白质因子RF作用机制的研究，该因子可逆转血红素缺乏的网织红细胞裂解物中的蛋白质合成抑制。
Proc Natl Acad Sci U S A. 1984 Sep;81(17):5379-83. doi: 10.1073/pnas.81.17.5379.
10
Protein synthesis in rabbit reticulocytes. A study of Met-tRNA f Met binding factor(s) and Met-tRNA f Met binding to ribosomes and AUG codon.兔网织红细胞中的蛋白质合成。对甲硫氨酰 - tRNAfMet 结合因子及甲硫氨酰 - tRNAfMet 与核糖体和 AUG 密码子结合的研究。
J Biol Chem. 1975 Feb 10;250(3):853-62.

引用本文的文献

1
Structurally and functionally modified forms of pp60v-src in Rous sarcoma virus-transformed cell lysates.劳氏肉瘤病毒转化细胞裂解物中pp60v-src的结构和功能修饰形式。
Mol Cell Biol. 1984 Jul;4(7):1213-20. doi: 10.1128/mcb.4.7.1213-1220.1984.
2
Tyrosine phosphorylation within the amino-terminal domain of pp60c-src molecules associated with polyoma virus middle-sized tumor antigen.与多瘤病毒中肿瘤抗原相关的pp60c-src分子氨基末端结构域内的酪氨酸磷酸化。
Proc Natl Acad Sci U S A. 1985 Jul;82(14):4568-72. doi: 10.1073/pnas.82.14.4568.
3
Occurrence of 40 S.polysomal complexes in polysome profiles of reticulocyte lysates.网织红细胞裂解液多核糖体图谱中40 S多核糖体复合物的出现情况。
Mol Biol Rep. 1988;13(1):53-8. doi: 10.1007/BF00805639.
4
Evidence for the presence of an inhibitor on ribosomes in mouse L cells infected with mengovirus.感染脑心肌炎病毒的小鼠L细胞核糖体上存在抑制剂的证据。
J Virol. 1985 Oct;56(1):161-71. doi: 10.1128/JVI.56.1.161-171.1985.

本文引用的文献

1
Isolation of a translational inhibitor from wheat germ with protein kinase activity that phosphorylates initiation factor eIF-2.从小麦胚芽中分离出一种具有蛋白激酶活性的翻译抑制剂，该蛋白激酶可使起始因子eIF-2磷酸化。
Biochem Biophys Res Commun. 1980 Dec 16;97(3):1124-32. doi: 10.1016/0006-291x(80)91492-8.
2
Regulation of protein synthesis in eukaryotes by the protein kinases that phosphorylate initiation factor eIF-2. Evidence for a common mechanism of inhibition of protein synthesis.磷酸化起始因子eIF-2的蛋白激酶对真核生物蛋白质合成的调控。蛋白质合成抑制的共同机制的证据。
FEBS Lett. 1980 Apr 7;112(2):211-5. doi: 10.1016/0014-5793(80)80182-7.
3
Initiation reactions in the mRNA-dependent reticulocyte lysate.依赖mRNA的网织红细胞裂解物中的起始反应。
Biochim Biophys Acta. 1982 Aug 30;698(2):218-21. doi: 10.1016/0167-4781(82)90139-7.
4
The regulation of initiation of mammalian protein synthesis.哺乳动物蛋白质合成起始的调控。
Prog Nucleic Acid Res Mol Biol. 1981;25:127-85. doi: 10.1016/s0079-6603(08)60484-5.
5
Inhibition of peptide initiation on reticulocyte ribosomes by edeine.伊短菌素对网织红细胞核糖体上肽链起始的抑制作用。
Eur J Biochem. 1971 Jul 15;21(1):31-41. doi: 10.1111/j.1432-1033.1971.tb01436.x.
6
Initiation of protein synthesis: evidence for messenger RNA-independent binding of methionyl-transfer RNA to the 40 S ribosomal subunit.蛋白质合成的起始：甲硫氨酰 - 转运RNA与40S核糖体亚基的不依赖信使RNA结合的证据。
J Mol Biol. 1973 May 25;76(3):379-403. doi: 10.1016/0022-2836(73)90511-1.
7
Evidence for specific inhibition of translocation of aminoacyl-transfer ribonucleic acid by the pretreatment of ribosomes of Bacillus subtilis with p-chloromercuribenzoate.对枯草芽孢杆菌核糖体用对氯汞苯甲酸预处理后，氨酰转移核糖核酸移位受到特异性抑制的证据。
J Bacteriol. 1972 Oct;112(1):188-94. doi: 10.1128/jb.112.1.188-194.1972.
8
An efficient mRNA-dependent translation system from reticulocyte lysates.一种来自网织红细胞裂解物的高效mRNA依赖性翻译系统。
Eur J Biochem. 1976 Aug 1;67(1):247-56. doi: 10.1111/j.1432-1033.1976.tb10656.x.
9
Regulation of protein synthesis in rabbit reticulocyte lysates: preparation of efficient protein synthesis lysates and the purification and characterization of the heme-regulated translational inhibitory protein kinase.兔网织红细胞裂解物中蛋白质合成的调控：高效蛋白质合成裂解物的制备以及血红素调节的翻译抑制蛋白激酶的纯化与特性分析
Methods Enzymol. 1979;60:459-84. doi: 10.1016/s0076-6879(79)60045-9.
10
Regulation of protein synthesis in eukaryotes.真核生物中蛋白质合成的调控
Annu Rev Biochem. 1979;48:549-80. doi: 10.1146/annurev.bi.48.070179.003001.

钒酸盐对真核生物蛋白质链起始的抑制作用。

Inhibition of eukaryotic protein chain initiation by vanadate.

作者信息

Ranu R S

出版信息

Proc Natl Acad Sci U S A. 1983 Jun;80(11):3148-52. doi: 10.1073/pnas.80.11.3148.

DOI:10.1073/pnas.80.11.3148
PMID:6574475
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC393997/
Abstract

Vanadate inhibits protein chain initiation in rabbit reticulocyte lysates. The evidence that supports this conclusion is as follows: (i) the biphasic kinetics of inhibition in which protein synthesis is maintained at the control rate for 1-2 min is followed by an abrupt decline in the rate of synthesis; (ii) inhibition is associated with a marked disaggregation of polyribosomes and a concomitant increase in 80S ribosomes; and (iii) vanadate concentrations that inhibit protein chain initiation do not inhibit polypeptide chain elongation or the aminoacylation of tRNA. In partial reactions of protein chain initiation, vanadate concentrations that inhibit protein synthesis have no detectable effect on the formation of eukaryotic initiation factor eIF-2-promoted ternary complex with Met-tRNAf and GTP and on the assembly of 40S ribosomal subunit-Met-tRNAf complexes. On the addition of mRNA, the 40S ribosomal subunit-Met-tRNAf complexes also are transformed into 80S ribosome-mRNA-Met-tRNAf complexes, termed 80S initiation complexes. In vanadate-treated samples, however, these 80S initiation complexes are defective and unable to proceed beyond this step.

摘要

钒酸盐抑制兔网织红细胞裂解物中的蛋白质链起始。支持这一结论的证据如下：（i）抑制的双相动力学，即蛋白质合成在1 - 2分钟内维持在对照速率，随后合成速率急剧下降；（ii）抑制与多核糖体的明显解聚以及80S核糖体的相应增加有关；（iii）抑制蛋白质链起始的钒酸盐浓度不抑制多肽链延伸或tRNA的氨酰化。在蛋白质链起始的部分反应中，抑制蛋白质合成的钒酸盐浓度对真核起始因子eIF - 2促进的与Met - tRNAf和GTP形成的三元复合物以及40S核糖体亚基 - Met - tRNAf复合物的组装没有可检测到的影响。加入mRNA后，40S核糖体亚基 - Met - tRNAf复合物也转化为80S核糖体 - mRNA - Met - tRNAf复合物，称为80S起始复合物。然而，在钒酸盐处理的样品中，这些80S起始复合物有缺陷，无法超越这一步骤。