Fu T K, Kessler J O, Jarvik L F, Matsuyama S S
Cell Biophys. 1982 Jun-Sep;4(2-3):77-95. doi: 10.1007/BF02918305.
A newly developed technique for quantitating the locomotion of polymorphonuclear leukocyte (PMN) populations in temperature gradients has revealed that PMNs accumulate toward higher temperatures. The experiments yield measurements of the numbers of cells that adhere to glass and migrate from a cell suspension through a liquid/gel meniscus into a glass/agarose interface, and of their spatial distribution at subsequent time intervals. Cell locomotion was investigated as a function of the magnitude, sign, and temporal variation of the temperature gradient, the cell concentration in the source suspension, and the presence or absence of chemoattractant gradients. It was found (1) that a temperature gradient stimulates crossing of the meniscus toward higher temperatures, (2) that only a portion of the cells reverses direction of locomotion in response to reversal of the temperature gradient after the cells have traversed the meniscus, and (3) that the distribution of cells in the migration space depends on cell concentration, suggesting that the dynamics of PMN locomotion depend on cell-cell interactions.
一种新开发的用于定量多形核白细胞（PMN）群体在温度梯度中运动的技术表明，PMN会向更高温度聚集。实验测量了附着在玻璃上并从细胞悬液通过液/凝胶弯月面迁移到玻璃/琼脂糖界面的细胞数量，以及它们在随后时间间隔的空间分布。研究了细胞运动与温度梯度的大小、符号和时间变化、源悬液中的细胞浓度以及趋化因子梯度的有无之间的关系。结果发现：（1）温度梯度会刺激细胞穿过弯月面朝向更高温度；（2）只有一部分细胞在穿过弯月面后，会因温度梯度的反转而改变运动方向；（3）迁移空间中细胞的分布取决于细胞浓度，这表明PMN运动的动力学取决于细胞间的相互作用。
