Swanson R A, Tracy R P, Katzmann J A, Wilson D M, Young D S
Clin Chem. 1982 Oct;28(10):2033-9.
In this sensitive radioimmunoassay for beta 2-microglobulin (beta 2-M) involving a commercially available monoclonal antibody, we used a second monoclonal antibody, produced in our laboratory, for affinity-chromatographic purification of beta 2-M. Both monoclonal antibodies bound to all of the charge isomers of beta 2-M identified by two-dimensional gel electrophoresis. Polyethylene glycol was used to separate the phases after incubation for 3 h at room temperature. Sensitivity was 0.25 ng of beta 2-M. Within-assay CVs were less than 5.4%, between-assay CVs less than 7.3%. Analytical recovery was 95-102%. The reference interval was 1.2-2.2 mg/L for 49 healthy subjects. The correlation coefficient for comparison with a polyclonal antibody assay was 0.997 for 44 patients. In a study correlating serum beta 2-M and creatinine concentrations with glomerular filtration rate for 50 patients, correlation coefficients for log-log transformed data were -0.94 and- 0.95, respectively. Concentrations of beta 2-M were increased in serum of patients with imparied renal function and also in patients with normal renal function who had disorders involving the immunologic response. We found no clear-cut advantage of measuring serum beta 2-M over serum creatinine in the estimation of glomerular filtration rate.
在这种使用市售单克隆抗体的β2-微球蛋白（β2-M）灵敏放射免疫测定法中，我们使用了在我们实验室制备的第二种单克隆抗体，用于β2-M的亲和色谱纯化。两种单克隆抗体均与二维凝胶电泳鉴定出的β2-M的所有电荷异构体结合。室温孵育3小时后，使用聚乙二醇进行相分离。灵敏度为0.25 ngβ2-M。批内变异系数小于5.4%，批间变异系数小于7.3%。分析回收率为95%-102%。49名健康受试者的参考区间为1.2-2.2 mg/L。44名患者与多克隆抗体测定法比较的相关系数为0.997。在一项对50名患者血清β2-M和肌酐浓度与肾小球滤过率相关性的研究中，对数-对数转换数据的相关系数分别为-0.94和-0.95。肾功能受损患者以及免疫反应紊乱但肾功能正常患者的血清中β2-M浓度升高。在估计肾小球滤过率方面，我们未发现测定血清β2-M比测定血清肌酐有明显优势。
