Jhanwar S C, Burns J P, Alonso M L, Hew W, Chaganti R S
Cytogenet Cell Genet. 1982;33(3):240-8. doi: 10.1159/000131760.
Using a method of chromosome preparation which yielded pachytene spermatocytes with exceptionally well spread bivalents, we undertook detailed chromomere analysis of each of the 22 autosomal bivalents stained with orcein or quinacrine. A maximum of 442 chromomeres were recognized and mapped at mid-pachytene. The chromomere maps of pachytene chromosomes presented here corresponded well with the high resolution banding maps of somatic chromosomes at the 850 band stage described in the International System for Human Cytogenetic Nomenclature (ISCN, 1981). Our observations of other features of the pachytene chromosomes, such as the parameters, also agree well with previous reports.
我们采用一种染色体制备方法，该方法能产生具有异常伸展良好的二价体的粗线期精母细胞，对用orcein或喹吖因染色的22条常染色体二价体中的每一条进行了详细的染色粒分析。在粗线期中期最多识别并绘制了442个染色粒。此处呈现的粗线期染色体的染色粒图谱与《国际人类细胞遗传学命名系统》（ISCN，1981）中描述的850条带阶段的体细胞染色体高分辨率带型图谱非常吻合。我们对粗线期染色体其他特征（如参数）的观察结果也与先前的报道非常一致。
