Hernandez-Verdun D, Derenzini M, Bouteille M
Chromosoma. 1982;85(4):461-73. doi: 10.1007/BF00327343.
The relative distribution of NOR proteins and chromatin fibers in the nucleoli was visualized in human cell line. The chromatin was revealed by a Feulgen-like procedure using osmium-ammine as DNA tracer. This selective staining was combined with NOR-silver staining. We provide morphological evidence for constant overlapping of the silver deposit sites with dispersed intranucleolar chromatin fibers. Silver stained proteins were sometimes observed in contact with the chromatin fibers, suggesting that at least some of the Ag-NOR proteins might be closely connected with the dispersed nucleolar DNA.
在人类细胞系中观察了核仁组织区（NOR）蛋白和染色质纤维的相对分布。通过使用锇氨作为DNA示踪剂的类似福尔根染色法来显示染色质。这种选择性染色与核仁组织区银染相结合。我们提供了形态学证据，证明银沉积位点与分散的核仁内染色质纤维持续重叠。有时观察到银染蛋白与染色质纤维接触，这表明至少一些银染核仁组织区蛋白可能与分散的核仁DNA紧密相连。
