Department of Biology and Biotechnology "L. Spallanzani", University of Pavia.
Eur J Histochem. 2024 Feb 23;68(1):3989. doi: 10.4081/ejh.2024.3989.
The Feulgen reaction has been the first specific method for detecting DNA available at light microscopy since 1924. However, a similar specific method was proposed for electron microscopy only 50 years later. Here, we discuss the problems encountered in finding the electrondense reagent capable of taking advantage of the extremely high resolution offered by electron microscopy as well as some applications of the method.
Feulgen 反应自 1924 年以来一直是在光学显微镜下检测 DNA 的第一种特异性方法。然而，类似的特异性方法直到 50 年后才被提出用于电子显微镜。在这里，我们讨论了在寻找能够利用电子显微镜提供的极高分辨率的电子致密试剂时遇到的问题，以及该方法的一些应用。
