新鲜与冷冻保存（-196摄氏度）的人血小板在回收率、存活率及隔离模式方面的比较研究

Comparative study on the percentage of recovery, survival and mode of sequestration of fresh and frozen preserved (-196 degrees C) human platelets.

作者信息

Milev A, Karakostov K, Nikolov C, Dimitrov L, Tzonev L, Tzvetkov T, Aleksiev N

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1982;109(3):507-12.

PMID:6182072

Abstract

Platelet concentrates amounting to 142 X 10(9) +/- 11 X 10(9) cells are prepared by cytopheresis from one liter of blood. Ten of the concentrates are labelled with 51Cr and reinfused autologously. The other ten concentrates are frozen with a controlled rate freezer in a medium containing 10% DMSO and 5% glucose for 7 days at -196 degrees C. The fresh platelets have a 51Cr 24 hrs. recovery of 68 +/- 7% and a survival rate (T 1/2) of 10 +/- 1 days. The frozen preserved platelets have a 51Cr 24 hrs. recovery of 54 +/- 6.1%, a T 1/2 of 8.1 days, and significantly increased liver sequestration. There is a correlation between the reversal reaction in vitro after hypotonic shock and the 51Cr 24 hrs. in vivo recovery of fresh platelets and the 51Cr 1st hr. recovery of frozen platelets.

摘要

通过血细胞分离术从一升血液中制备出数量为142×10⁹±11×10⁹个细胞的浓缩血小板。其中十份浓缩血小板用⁵¹Cr标记后自体回输。另外十份浓缩血小板用程序降温冷冻仪在含有10%二甲基亚砜和5%葡萄糖的培养基中于-196℃冷冻7天。新鲜血小板的⁵¹Cr 24小时回收率为68±7%，存活率（T 1/2）为10±1天。冷冻保存的血小板的⁵¹Cr 24小时回收率为54±6.1%，T 1/2为8.1天，并且肝脏潴留显著增加。低渗休克后体外的逆转反应与新鲜血小板的⁵¹Cr 24小时体内回收率以及冷冻血小板的⁵¹Cr第1小时回收率之间存在相关性。