Du Boulay C E
Hum Pathol. 1982 Oct;13(10):925-9. doi: 10.1016/s0046-8177(82)80053-1.
Formalin-fixed, paraffin-embedded jejunal, lymph nodal, and splenic tissues were obtained from four patients with Whipple's disease. Using antisera to a variety of bacteria, sections were stained by the immunoperoxidase technique. Positive staining was obtained with antiserum to streptococcus group B in all tissues examined. The distinctive pattern of staining was similar to the findings of previous workers using fluorescein-labeled antibodies. However, when rigorous controls for specificity were applied in this study, it was not possible to confirm the presence of streptococcus B antigen in the tissues. The discrepancy between the specificity of the technique on control and test sections is interesting and emphasizes the need for adequate controls when the immunoperoxidase technique is used to look for microorganisms in tissues. It is suggested that the unknown microorganism observed in the macrophages of patients with Whipple's disease has been altered in some way to resemble streptococcus B antigenically or that it shares common antigens with streptococcus B.
从4例惠普尔病患者身上获取了用福尔马林固定、石蜡包埋的空肠、淋巴结和脾脏组织。使用针对多种细菌的抗血清，通过免疫过氧化物酶技术对切片进行染色。在所检查的所有组织中，用B族链球菌抗血清获得了阳性染色。这种独特的染色模式与先前使用荧光素标记抗体的研究结果相似。然而，在本研究中应用严格的特异性对照时，无法证实在组织中存在B族链球菌抗原。该技术在对照切片和测试切片上的特异性差异很有意思，强调了在使用免疫过氧化物酶技术在组织中寻找微生物时进行充分对照的必要性。有人提出，在惠普尔病患者巨噬细胞中观察到的未知微生物已在某种程度上发生改变，在抗原性上类似于B族链球菌，或者它与B族链球菌有共同抗原。
