一种用于抗半抗原抗体的改良酶联免疫吸附测定技术。
A modified ELISA technique for anti-hapten antibodies.
作者信息
Suter M
出版信息
J Immunol Methods. 1982 Aug 27;53(1):103-8. doi: 10.1016/0022-1759(82)90244-7.
Polystyrene tubes pretreated with glutaraldehyde in phosphate buffer were used in a solid-phase enzyme-linked immunoassary (ELISA). The hapten o-dinitrocarboxyphenol (o-DNCP) was used as coat. The binding of homologous rabbit antibodies was measured with phosphatase-labelled staphylococcal protein A (SPA-PH). This method is both very sensitive (serum dilutions greater than 2 x 10(-6) were still positive) and highly reproducible. Anti-DNP rat IgE was detected with o-DNCP hapten as a coat using avidin/biotin as a marker.
在固相酶联免疫吸附测定（ELISA）中使用经磷酸盐缓冲液中戊二醛预处理的聚苯乙烯管。半抗原邻二硝基羧基苯酚（o-DNCP）用作包被物。用磷酸酶标记的葡萄球菌蛋白A（SPA-PH）测定同源兔抗体的结合。该方法既非常灵敏（血清稀释度大于2×10⁻⁶仍为阳性）又具有高度可重复性。以o-DNCP半抗原作为包被物，使用抗生物素蛋白/生物素作为标记物检测抗DNP大鼠IgE。
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