通过对N-1位腺嘌呤进行氧化对真细菌5S核糖体RNA进行比较结构分析。

Comparative structural analysis of eubacterial 5S rRNA by oxidation of adenines in the N-1 position.

作者信息

Pieler T, Schreiber A, Erdmann V A

出版信息

Nucleic Acids Res. 1984 Apr 11;12(7):3115-26. doi: 10.1093/nar/12.7.3115.

DOI:10.1093/nar/12.7.3115

PMID:6201825

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318733/

Abstract

Adenines in free 5S rRNA from Escherichia coli, Bacillus stearothermophilus and Thermus thermophilus have been oxidized at their N-1 position using monoperphthalic acid. The determination of the number of adenine 1-N-oxides was on the basis of UV spectroscopic data of the intact molecule. Identification of the most readily accessible nucleotides by sequencing gel analysis reveals that they are located in conserved positions within loops, exposed hairpin loops and single-base bulge loops. Implications for the structure and function of 5S rRNA will be discussed on the basis of this comparative analysis.

摘要

来自大肠杆菌、嗜热脂肪芽孢杆菌和嗜热栖热菌的游离5S核糖体RNA中的腺嘌呤已使用单过氧邻苯二甲酸在其N-1位置被氧化。腺嘌呤1-N-氧化物数量的测定基于完整分子的紫外光谱数据。通过测序凝胶分析鉴定最易接近的核苷酸，结果表明它们位于环内、暴露的发夹环和单碱基凸起环内的保守位置。将基于这一比较分析讨论其对5S核糖体RNA结构和功能的影响。