Ares M, Howell S H
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5577-81. doi: 10.1073/pnas.79.18.5577.
The accumulation pattern of a number of mRNAs during the cell cycle of Chlamydomonas was examined by two-dimensional gel analysis of in vitro translation products and by RNA blot hybridization analysis. Two-dimensional gel analysis revealed that 10-15% of the 300 most abundant translation products are differentially synthesized from RNA obtained at various cell cycle stages. RNAs that direct the synthesis of alpha- and beta-tubulins and that hybridize to cloned alpha- and beta-tubulin probes accumulate coordinately during the predivision period of the cell cycle, reaching peak levels before or during division. Other RNAs represented by selected cloned cDNA probes show a number of different cell cycle patterns of accumulation. The accumulation patterns of these RNAs are not directly influenced by ongoing illumination conditions, even though alternating light-dark illumination cycles are used to synchronize Chlamydomonas cells. The results suggest that there may be a complex program of gene expression correlated with cell cycle progression in Chlamydomonas.
通过对体外翻译产物的二维凝胶分析以及RNA印迹杂交分析，研究了衣藻细胞周期中多种mRNA的积累模式。二维凝胶分析表明，300种最丰富的翻译产物中，有10% - 15%是由不同细胞周期阶段获得的RNA差异合成的。指导α-和β-微管蛋白合成并与克隆的α-和β-微管蛋白探针杂交的RNA，在细胞周期的分裂前期协同积累，在分裂前或分裂期间达到峰值水平。由选定的克隆cDNA探针代表的其他RNA显示出多种不同的细胞周期积累模式。这些RNA的积累模式不受持续光照条件的直接影响，即使使用明暗交替光照周期来同步衣藻细胞。结果表明，衣藻中可能存在与细胞周期进程相关的复杂基因表达程序。
