Bigl V, Woolf N J, Butcher L L
Brain Res Bull. 1982 Jun;8(6):727-49. doi: 10.1016/0361-9230(82)90101-0.
The morphologies, intercellular organization, and cortical projection patterns of putative cholinergic neurons in the basal forebrain of the rat were examined by use of fluorescent tracer histology in combination with the pharmacohistochemical regimen for acetylcholinesterase (AChE). Intensity staining AChE-containing cells projecting to frontal sensorimotor (Area 10), parietal (Area 2), and temporal (Area 4) cortices were found ipsilaterally in nucleus preopticus magnocellularis, in nucleus basalis, and in association with the substantia innominata, the ansa lenticularis, and the lateral hypothalamic area; an essentially rostrocaudal topography was observed for these projections. AChE-containing pathways to cingulate (Area 29) and visual (Area 17) cortices derived from ipsilateral somata associated with the vertical and horizontal limbs of the diagonal band, nucleus preopticus magnocellularis, rostral portions of nucleus basalis, and the substantia innominata. Neurons innervating Area 29 were generally located more rostrally than those giving rise to AChE afferents to Area 17. The vast majority of cells appeared to innervate relatively discrete areas of the cortex. Evidence for collateralization was found only in neurons projecting to visual and cingulate cortices, and these represented only 3.2% of the cells providing AChE afferents to Areas 17 and 29. The basal forebrain AChE projection cells were typically large (greater than 25 micron in maximum cell body extent), and their somata were predominantly oval, with lesser proportions being fusiform or triangular. Many were organized in clusters, particularly in nucleus basalis.
通过荧光示踪组织学结合乙酰胆碱酯酶（AChE）的药物组织化学方法，研究了大鼠基底前脑假定胆碱能神经元的形态、细胞间组织和皮质投射模式。在视前大细胞核、基底核以及与无名质、豆状袢和下丘脑外侧区相关的区域同侧，发现了向额叶感觉运动区（10区）、顶叶（2区）和颞叶（4区）皮质投射的强AChE染色细胞；观察到这些投射基本呈前后向的拓扑结构。向扣带回（29区）和视觉皮质（17区）的含AChE通路源自同侧的胞体，这些胞体与斜角带的垂直和水平支、视前大细胞核、基底核的前部以及无名质相关。支配29区的神经元通常比那些向17区发出AChE传入纤维的神经元位置更靠前。绝大多数细胞似乎支配皮质相对离散的区域。仅在向视觉和扣带回皮质投射的神经元中发现了侧支化的证据，这些神经元仅占向17区和29区提供AChE传入纤维的细胞的3.2%。基底前脑的AChE投射细胞通常较大（最大细胞体直径大于25微米），其胞体主要呈椭圆形，较少呈梭形或三角形。许多细胞成簇排列，尤其是在基底核中。
