van Toorenenbergen A W, Aalberse R C
Clin Allergy. 1982 Sep;12(5):451-8. doi: 10.1111/j.1365-2222.1982.tb01643.x.
Leucocytes from normal non-allergic donors were passively sensitized with sixty-five atopic sera that contained IgE directed against house dust mite (HDM) or guinea-pig dander (GPD). In no serum was there any IgE with an abnormal basophil anaphylactic-sensitizing activity. In sera from patients hypersensitive to GPD, both GPD-specific IgE and IgG4 were measured. A significant correlation was found between the GPD-IgE RAST and the basophil-sensitizing capacity of GPD-positive sera, but no correlation was found between this sensitizing capacity and the GPD-IgG4 RAST. Leucocytes were passively sensitized with serum mixtures that contained the same amounts of total and HDM-specific IgE; these mixtures differed as to sensitizing activity. It is unclear whether this variation is due to differences in IgE or to influences of other factors in serum.
来自正常非过敏供体的白细胞用65份特应性血清进行被动致敏，这些血清含有针对屋尘螨（HDM）或豚鼠皮屑（GPD）的IgE。在任何血清中均未发现具有异常嗜碱性粒细胞过敏致敏活性的IgE。在对GPD过敏的患者血清中，同时检测了GPD特异性IgE和IgG4。发现GPD-IgE RAST与GPD阳性血清的嗜碱性粒细胞致敏能力之间存在显著相关性，但该致敏能力与GPD-IgG4 RAST之间未发现相关性。用含有相同总量和HDM特异性IgE的血清混合物对白细胞进行被动致敏；这些混合物在致敏活性方面存在差异。目前尚不清楚这种差异是由于IgE的不同还是血清中其他因素的影响。
