Weyer A, Ougen P, Dandeu J P, Marchand F, David B
Agents Actions. 1986 Apr;18(1-2):178-81. doi: 10.1007/BF01988015.
Basophils from allergic or non-atopic donors were depleted for their native membrane IgE by acid treatment and then passively sensitized by Dermatophagoides farinae specific IgE containing sera. Histamine release experiments were performed with a highly purified allergen (Df 11) on native cells, acid treated cells and passively sensitized cells. In the sensitization procedure, the quantity of the basophil-bound serum IgE is dependent on the concentration of the sensitizing serum IgE and the histamine release capacity is specifically acquired. It is shown that after passive sensitization basophil membrane IgE density as well as basophil sensitivity to Df 11 in histamine release experiments depend on the ratio [Df 11 specific IgE/total IgE] in the sensitizing serum.
来自过敏或非特应性供体的嗜碱性粒细胞经酸处理去除其天然膜IgE，然后用含有粉尘螨特异性IgE的血清进行被动致敏。用高度纯化的变应原（Df 11）对天然细胞、酸处理细胞和被动致敏细胞进行组胺释放实验。在致敏过程中，嗜碱性粒细胞结合的血清IgE量取决于致敏血清IgE的浓度，并且组胺释放能力是特异性获得的。结果表明，在被动致敏后，组胺释放实验中嗜碱性粒细胞膜IgE密度以及嗜碱性粒细胞对Df 11的敏感性取决于致敏血清中的[Df 11特异性IgE/总IgE]比值。
