Huskinson J, Stepick-Biek P N, Araujo F G, Thulliez P, Suzuki Y, Remington J S
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.
J Clin Microbiol. 1989 Sep;27(9):2031-8. doi: 10.1128/jcm.27.9.2031-2038.1989.
The immunoglobulin G (IgG) subclass response to Toxoplasma gondii antigens during the acute and chronic stages of T. gondii infection were studied by using immunoblots with reduced antigen (RA) and nonreduced antigen (NRA) preparations. Serum samples were from individuals with acute or chronic T. gondii infection, and sequential samples were from women who seroconverted during gestation and were treated with spiramycin. IgG1 antibodies were predominant in sera from each of the groups and recognized a large number of RA and NRA. In the latter group of patients, IgG1 and IgG3 were the first antibodies to appear in response to the infection. In all groups, an antigen with a molecular weight (MW) of 30,000 was the most intensely stained and frequently recognized by IgG1 antibodies in NRA preparations. In RA preparations, antigens of MW 35,000 and 30,000 were the most intensely stained and frequently recognized by IgG1 antibodies. An antigen with an MW of 22,000 was intensely stained in the IgG1 immunoblots of the NRA preparation and to a lesser extent in the RA preparation. In contrast to immunoblots with IgG1 antibodies, very few antigens in the RA and NRA preparations were detected by IgG2 and IgG3 antibodies; IgG4 antibodies rarely detected any antigens. Of interest was that IgG2 antibodies detected antigens distributed over the entire MW range, whereas those detected by IgG3 antibodies were located mostly below the 35,000 MW marker. Enzyme-linked immunosorbent assay results paralleled those of the immunoblots in that IgG1 antibodies were predominant.
通过使用还原抗原（RA）和非还原抗原（NRA）制剂的免疫印迹法，研究了弓形虫感染急性期和慢性期对弓形虫抗原的免疫球蛋白G（IgG）亚类反应。血清样本来自急性或慢性弓形虫感染个体，连续样本来自孕期血清学转换并接受螺旋霉素治疗的女性。IgG1抗体在每组血清中占主导地位，并识别大量的RA和NRA。在后一组患者中，IgG1和IgG3是感染后最早出现的抗体。在所有组中，分子量（MW）为30,000的抗原在NRA制剂中染色最深，且最常被IgG1抗体识别。在RA制剂中，MW为35,000和30,000的抗原染色最深，且最常被IgG1抗体识别。MW为22,000的抗原在NRA制剂的IgG1免疫印迹中染色强烈，在RA制剂中染色程度较轻。与IgG1抗体的免疫印迹相反，RA和NRA制剂中很少有抗原被IgG2和IgG3抗体检测到；IgG4抗体很少检测到任何抗原。有趣的是，IgG2抗体检测到的抗原分布在整个MW范围内，而IgG3抗体检测到的抗原大多位于MW 35,000标记以下。酶联免疫吸附测定结果与免疫印迹结果相似，即IgG1抗体占主导地位。
