Lemonnier F A, Rebai N, Le Bouteiller P P, Malissen B, Caillol D H, Kourilsky F M
J Immunol Methods. 1982 Oct 15;54(1):9-22. doi: 10.1016/0022-1759(82)90108-9.
A solid-phase radioimmunoanalysis of plasma membrane molecules solubilized in the presence of detergent was developed. From a crude cell lysate, membrane molecules were specifically immobilized by solid-phase adsorbed monoclonal antibodies. Analysis of these molecules was easily performed with radiolabeled monoclonal antibodies of different specificities. This technique was used to analyze the HLA-DR and HLA-A,B,C molecules expressed by RAJI cells. It was possible (a) to determine which epitopes are expressed on the same molecules; (b) to define subsets of HLA molecules according to the epitopes which they express, and (c) to perform quantitative analysis of HLA molecules on a crude cell lysate.
开发了一种对在去污剂存在下溶解的质膜分子进行的固相放射免疫分析方法。从粗制细胞裂解物中，膜分子通过固相吸附的单克隆抗体特异性固定。使用具有不同特异性的放射性标记单克隆抗体可轻松对这些分子进行分析。该技术用于分析RAJI细胞表达的HLA-DR和HLA-A、B、C分子。有可能（a）确定哪些表位在同一分子上表达；（b）根据它们表达的表位定义HLA分子亚群，以及（c）对粗制细胞裂解物中的HLA分子进行定量分析。
