新生大鼠肝脏金属硫蛋白cDNA的克隆

Cloning of metallothionein cDNA from neonatal rat liver.

作者信息

Mercer J F, Hudson P

出版信息

Biosci Rep. 1982 Oct;2(10):761-8. doi: 10.1007/BF01114935.

Abstract

A metallothionein cDNA clone was isolated from a cDNA bank prepared from neonatal rat liver poly(A)-containing RNA by a colony screening procedure using [32P]cDNA probes prepared from mRNA of either metal-induced or uninduced rat livers. Nucleotide sequence analysis of this clone showed that it contained the entire 3' untranslated region and 30% of the coding sequence for a rat metallothionein. The sequence is remarkably homologous with the mouse metallothionein-I gene.

摘要

通过使用从金属诱导或未诱导的大鼠肝脏mRNA制备的[32P]cDNA探针，采用菌落筛选程序，从新生大鼠肝脏含多聚腺苷酸（poly(A)）的RNA制备的cDNA文库中分离出一个金属硫蛋白cDNA克隆。对该克隆的核苷酸序列分析表明，它包含大鼠金属硫蛋白完整的3'非翻译区和30%的编码序列。该序列与小鼠金属硫蛋白-I基因具有显著的同源性。