Saijoh K, Kuno T, Shuntoh H, Tanaka C, Sumino K
Department of Public Health, Kobe University School of Medicine, Japan.
Pharmacol Toxicol. 1989 May;64(5):464-8. doi: 10.1111/j.1600-0773.1989.tb00688.x.
A rat brain metallothionein-II (MT-II) complementary DNA (cDNA) clone was isolated from a cDNA plasmid library, which was prepared from non-treated rat brain mRNA, by a colony screening procedure using 32P-labeled synthetic oligonucleotide probes. It is deduced that the clone encodes for a protein of 61 amino acids comprising 20 cysteines, which is highly homologous to MT-IIs in other species. Northern blot analysis demonstrated major mRNA species in the brain, liver and kidneys (approximately 350 b in size), which is induced in response to dexamethasone, zinc, cadmium and mercury but not to methyl mercury. These findings confirm that MT-II genes are expressed and regulated both by steroid and heavy metals in the brain as well as in peripheral organs.
从非处理大鼠脑mRNA制备的cDNA质粒文库中，通过使用32P标记的合成寡核苷酸探针的菌落筛选程序，分离出大鼠脑金属硫蛋白-II（MT-II）互补DNA（cDNA）克隆。据推测，该克隆编码一种由61个氨基酸组成的蛋白质，其中包含20个半胱氨酸，与其他物种的MT-II高度同源。Northern印迹分析表明，脑、肝和肾中存在主要的mRNA种类（大小约为350 b），其可被地塞米松、锌、镉和汞诱导，但不能被甲基汞诱导。这些发现证实，MT-II基因在脑以及外周器官中均受类固醇和重金属的表达调控。
