Rojas A M, Steinsapir J
Endocrinology. 1983 Feb;112(2):586-91. doi: 10.1210/endo-112-2-586.
Eosinophils appear in the rat uterus in the presence of estrogen. The level of these cells in the uterus depends on the number of blood eosinophils. Insulin is an eosinopenic hormone in the blood and, therefore, could regulate estrogenic responses mediated by these cells in the uterus. Estrogen-induced uterine edema and eosinophilia at doses of 0.01,. 0.1, 1, 10, and 30 micrograms 17 beta-estradiol (E2)/100 g BW are inhibited by insulin. Estrogen binding by uterine eosinophils in vitro decreases in the presence of insulin, suggesting another explanation for the observations in the uterus in vivo. Injection of insulin alone or in combination with 0.01, 0.01, 0.1, or 1 microgram E2/100 g BW increases uterine RNA and protein contents by 6 h. Inactive insulin does not modify any of these stimulatory effects of estrogen. The results support the idea of two separate receptor systems for estrogens in the rat uterus: the eosinophil receptor system, which mediates estrogen-induced uterine edema, and the cytosol-nuclear receptor system, which mediates estrogen-induced uterine RNA and protein syntheses.
在雌激素存在的情况下，嗜酸性粒细胞会出现在大鼠子宫中。子宫中这些细胞的水平取决于血液中嗜酸性粒细胞的数量。胰岛素是血液中的一种嗜酸性粒细胞减少激素，因此可能调节这些细胞在子宫中介导的雌激素反应。胰岛素可抑制剂量为0.01、0.1、1、10和30微克17β-雌二醇（E2）/100克体重时雌激素诱导的子宫水肿和嗜酸性粒细胞增多。在胰岛素存在的情况下，子宫嗜酸性粒细胞在体外的雌激素结合减少，这为体内子宫中的观察结果提供了另一种解释。单独注射胰岛素或与0.01、0.01、0.1或1微克E2/100克体重联合注射6小时后，子宫RNA和蛋白质含量增加。无活性胰岛素不会改变雌激素的任何这些刺激作用。结果支持大鼠子宫中存在两种独立的雌激素受体系统的观点：介导雌激素诱导的子宫水肿的嗜酸性粒细胞受体系统，以及介导雌激素诱导的子宫RNA和蛋白质合成的胞质-核受体系统。
