Hagberg M, Ahlstedt S, Hanson L
Eur J Clin Microbiol. 1982 Feb;1(1):59-65. doi: 10.1007/BF02014142.
Antibodies against Escherichia coli O antigen from rabbits immunized with formalin-killed bacteria were tested for cytotoxic capacity in an antibody-dependent cell-mediated cytotoxicity (ADCC) assay with human lymphocytes as effector cells and autologous papainized erythrocytes coated with O antigen as target cells. The cytotoxic titres were compared with the titres obtained with three methods of antibody quantitation. It was found that ADCC recorded antibodies with similar sensitivity as the enzyme-linked immunosorbent assay (ELISA) for IgG, but was much more sensitive than the ammonium sulphate precipitation (ASP) and indirect haemagglutination (IHA) using beta-mercaptoethanol reduced sera. The ADCC titres were found to correlate very well with the titres obtained with ASP, ELISA and IHA for IgG but not for IgM, which is in accordance with a previous notion that ADCC is primarily mediated via IgG antibodies. ADCC should be considered as a possible immunopathologic mechanism in renal parenchymal damage in connection with urinary tract infections.
用福尔马林灭活细菌免疫的兔抗大肠杆菌O抗原抗体，在以人淋巴细胞为效应细胞、以包被O抗原的自体木瓜蛋白酶处理红细胞为靶细胞的抗体依赖性细胞介导的细胞毒性（ADCC）试验中检测其细胞毒性能力。将细胞毒性滴度与三种抗体定量方法获得的滴度进行比较。结果发现，ADCC检测抗体的灵敏度与酶联免疫吸附测定（ELISA）检测IgG的灵敏度相似，但比使用β-巯基乙醇还原血清的硫酸铵沉淀法（ASP）和间接血凝法（IHA）灵敏得多。发现ADCC滴度与ASP、ELISA和IHA检测IgG的滴度相关性很好，但与IgM的滴度无关，这与之前认为ADCC主要由IgG抗体介导的观点一致。ADCC应被视为与尿路感染相关的肾实质损伤中一种可能的免疫病理机制。
